General Cell Based Assays
Eurofins Discoverx PathHunter eXpress GPR88 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0357E2ACP1L)
The PathHunter GPR88 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR88 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR88 and EA-tagged β-Arrestin-2. Activation of GPR88 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eagle Biosciences Inc Creatinine Microplate Assay Kit
Creatinine Assay Kit (urine normalizing assay)
Eurofins Discoverx AssayComplete Cell Plating 23 Reagent, 100. (93-0563R23A)
AssayComplete Cell Plating 23 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx AssayComplete Cell Plating 38 Reagent, 100. (93-0563R38A)
AssayComplete Cell Plating 38 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress ADRB1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0488E2CP0M)
The PathHunter ADRB1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. isoproterenol) based activation of ADRB1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged ADRB1 and EA-tagged β-Arrestin-2. Activation of ADRB1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress ADORA3 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0586E2CP2M)
The PathHunter ADORA3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. MECA) based activation of ADORA3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged ADORA3 and EA-tagged β-Arrestin-2. Activation of ADORA3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress FPR1 Total Internalization Assay, 10pl Kit. (93-0873E3CP0L)
The PathHunter FPR1 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of FPR1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged FPR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the FPR1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress ADRA2A CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0424E2CP0M)
The PathHunter ADRA2A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA2A GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged ADRA2A and EA-tagged β-Arrestin-2. Activation of ADRA2A stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mEDNRB CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0602E2MCP0M)
The PathHunter mEDNRB eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. endothelin-3) based activation of mEDNRB GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mEDNRB and EA-tagged β-Arrestin-2. Activation of mEDNRB stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress rTrkA-rP75 Functional Assay, 2-Plate Kit. (93-1014E3CP16M)
The PathHunter eXpress rTrkA-rP75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated rTrkA and rP75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged rTrkA and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, rP75 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress GPR109A CHO-K1 GPCR Assay, 2-Plate Kit. (95-0122E2CP2M)
The cAMP Hunter GPR109A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. nicotinic acid) based activation of GPR109A GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GPR109A, a naturally Gi/Go coupled GPCR. Upon activation of GPR109A, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress OPRM1 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0107E2CP2M)
The cAMP Hunter OPRM1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. met-enkephalin, morphine) based activation of OPRM1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type OPRM1, a naturally Gi/Go coupled GPCR. Upon activation of OPRM1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress EphB1 Functional Assay, 2-Plate Kit. (93-0893E3CP16M)
The PathHunter eXpress EphB1 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated EphB1 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged EphB1 and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
TRIBIOSCIENCE INC Cytochrome C Reductase Activity Assay 100 Tests
NADPH-cytochrome C reductase is widely used as an endoplasmic reticulum marker and as a biomarker of ecological pollution and dietary lipid uptake. This kit is based on a colorimetric assay that measures the reduction of cytochrome C by NADPH-Cytochrome C reductase in the presence of NADPH. The reduction of cytochrome C results in the formation of distinct bands in the absorption spectrum and the increase in absorbance at 550 nm is measured with time.
Eurofins Discoverx PathHunter eXpress UTR2 Total GPCR Internalization Assay, 2-Plate Kit. (93-0746E3CP5M)
The PathHunter UTR2 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of UTR2 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged UTR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the UTR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.