General Cell Based Assays
Abcam Alpha Galactosidase Activity Assay Kit
Alpha Galactosidase Activity Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress GPR25 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0368E2ACP2M)
The PathHunter GPR25 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR25 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR25 and EA-tagged β-Arrestin-2. Activation of GPR25 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical Cell-Based Assay Buffer Tablet, 4 ea
Cell-Based Assay Buffer Tablet has been formulated and tested to work with Cayman’s Assay Kits. Detailed instructions for its use are contained in the respective Cayman Assay Kit booklet.
Abcam NETosis Assay Kit
NETosis Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical Estradiol 17-(β-D-Glucuronide) (sodium salt hydrate), Purity: ≥95%, 25 mg
An estrogen metabolite that acts as a substrate of MRP2 (Km = 75 µM), and through MRP2-mediated transport, functions as a cholestatic agent, decreasing bile flow
Eurofins Discoverx InCELL Hunter eXpress Bcl2L1-Bax Protein Binding Assay, 2-plate Kit. (96-0009E3CP16M)
The InCELL Hunter eXpress Bcl2L1-Bax Protein Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that disrupt protein binding. This kit assesses compound-based disruption of Bcl2L1-Bax binding via detection of Bax levels. This product uses EFC technology. The cell line included overexpresses Bcl2L1 target in an untagged form and Bax binding partner fused to ProLabel. When a compound engages the Bcl2L1 target protein, partner binding is disrupted and the destabilized Bax is degraded, leading to a loss of EFC signal upon addition of EA containing detection reagent. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Medchemexpress LLC TrkC Human HEK293 50ug
TrkC Protein a receptor tyrosine kinase crucially contributes to nervous system and potential heart development Upon NTF3/neurotrophin-3 binding TrkC undergoes autophosphorylation activating pathways like phosphatidylinositol 3-kinase/AKT and MAPK These pathways regulate cell survival and differentiation emphasizing TrkC s pivotal role in essential cellular processes during development TrkC Protein Human (HEK293 His) is the recombinant human-derived TrkC protein expressed by HEK293 with C-His labeled tag
ABclonal Technology CAMP Rabbit pAb
This gene encodes a member of an antimicrobial peptide family, characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide, which is produced by extracellular proteolysis from the C-terminus. The protein plays an important role in innate immunity defense against viruses. In addition to its antibacterial, antifungal, and antiviral activities, the encoded protein functions in cell chemotaxis, immune mediator induction, and inflammatory response regulation.
American Research Products Inc NF-kappaB p65 (Phospho-Ser276) Transcription Factor Activity Assay
NF-kappaB p65 (Phospho-Ser276) Transcription Factor Activity Assay
Eurofins Discoverx cAMP Hunter eXpress GALR1 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0058E2CP2M)
The cAMP Hunter GALR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. galanin) based activation of GALR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GALR1, a naturally Gi/Go coupled GPCR. Upon activation of GALR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF cAMP Gi Detection Kit, 20,000 Assay Points
HTRF cAMP Gi Detection Kit, 20,000 Assay Points
Meso Scale Discovery R-PLEX Human MET soluble ASY
The R-PLEX Human Met Soluble Assay from Meso Scale Discovery is a multiplex immunoassay that measures 10 metabolic biomarkers in human serum plasma and cell culture supernatant It uses electrochemiluminescence detection for high sensitivity and broad dynamic range with low sample volume The panel includes insulin leptin PAI-1 total resistin MCP-1 adiponectin TNF-alpha IL-6 CRP and visfatin The assay provides efficient analysis of metabolic and inflammatory status with a protocol time of just over 3 hours It includes a 96-well plate with capture antibodies detection antibodies and calibrators For research use only
Eurofins Discoverx PathHunter eXpress IL2RB/IL2RG Dimerization Assay, 10pl Kit. (93-0998E3CP5L)
The PathHunter IL2RB/IL2RG Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-2) induced dimerization of IL2RB/IL2RG receptors. This product uses EFC technology. The cell line included overexpresses IL2RB target protein fused to PK alongside IL2RG target protein fused to EA. Upon ligand-induced dimerization of IL2RB/IL2RG, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Bioassay Systems EnzyChrom™ Superoxide Dismutase Assay Kit. For quantitative determination of superoxide dismutase (SOD) enzyme activity.
For quantitative determination of superoxide dismutase (SOD) enzyme activity and evaluation of drug effects. Key Features: Sensitive and accurate. Linear detection range of 0.05 - 3 U/mL SOD. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD440nm. Samples: Blood, cell, tissue etc. Species: All. Procedure: Assay takes 1 hr. Kit size: 100 tests. Detection limit: 0.05 U/mL.
Epigentek Inc Epigenase HDAC Activity/Inhibition Direct Assay Kit (Colorimetric) (48 assays)
The Epigenase HDAC Activity/Inhibition Direct Assay Kit is a complete set of optimized buffers and reagents for measuring the activity or inhibition of total HDAC enzymes in nuclear extracts or purified HDAC isoforms (HDACs 1-11) from a broad range of species such as mammals, plants, fungi, and bacteria, and in a variety of forms including, but not limited to, cultured cells and fresh and frozen tissues. The Epigenase HDAC Activity/Inhibition Direct Assay Kit is a further refinement of its predecessor, the EpiQuik HDAC Activity/Inhibition Assay Kit, a popular assay method for HDAC activity and inhibition. This latest method retains the simplicity, rapidness, high throughput capability, and non-radioactivity featured in the previous version.